Checklist *Title and abstract (a) Indicate the study’s design with a commonly used term in the title or the abstract (b) Provide in the abstract an informative and balanced summary of what was done and what was found *Introduction -Background/rationale Explain the scientific background and rationale for the investigation being reported -Objectives State specific objectives, including any research questions and prespecified hypotheses *Methods -Study design Present key elements of study design early in the paper -Study setting Describe the setting, locations, and relevant dates, including periods of recruitment, exposure, follow-up, and data collection. Use a flow diagram if necessary. *Participants Provide characteristics of study participants (eg demographic, clinical, social) Cross-sectional study—Give the eligibility criteria, and the sources and methods of selection of participants *Variables Clearly define all outcomes, exposures, predictors, potential confounders, and effect modifiers. Give diagnostic criteria, if applicable *Data sources/ measurement For each variable of interest, give sources of data and details of methods of assessment (measurement). Describe comparability of assessment methods if there is more than one group *Bias Describe any efforts to address potential sources of bias *Statistical methods (a) Describe all statistical methods, including those used to control for confounding *Results -Main results Provide expected results based on hypotheses. Include how measures of disease frequency and comparison will be interpreted. Include any tables as necessary.