Go to the following link and complete the microarray and PCR activities. Answer the following questions. It will be much easier to answer the questions while you are completing the lab activities.

 1.       How many chemical base pairs are in the human genome?

 

 

2.      
What is the purpose of PCR?

 

 

 

3.      
What is the function of the primer?

 

 

4.      
What are nucleotides?

 

 

5.      
What is the last step of the experiment?

 

6.      
What is the DNA thermal cycler?

 

 

7.      
What happens at 203⁰F?

 

 

8.      
What happens at 122⁰F? 

1.      
What happens at 72⁰C?

 

 

2.      
In cycle 2, why is the temperature raised?

 

 

3.      
What are the desired products of cycle 3?

 

4.      
After 30 cycles, what is your product?

 

Complete the experiment, answer these questions

5.      
 You are a researcher interested studying genes that have an increased level of activity in skin cancer cells, what color spots should you choose?

 

 

6.      
What is the job of gene4263?

 

 

7.      
What color are the spots regulated by gene4263?

 

 

8.      
What color is represented by 6219?

 

 

9.      
What does RNA expression usually indicate?

 

10.  
Why is gene6219 the color it is?

 

 

11.  
Why can’t 6219 be detected in the microarray analysis?

 

 

12.  
List 3 things microarray analysis cannot do?